Fersht, A. 45, 273-387. Membrane trafficking. John R. Engen and Ioan Lascu are acknowledged for their participation, encouragement and support in the realization of several of the presented studies. J. Biol. Chem. Seaki, T., Hori, M., and Umezawa, H. (1974). An official website of the United States government. Khan, I.; Steeg, P.S. Janus-faces of NME-oncoprotein interactions. ; Boissan, M.; Lacombe, M.L. Cleland, W. W., and Northrop, D. B. Biochem. ; Liotta, L.A.; Sobel, M.E. Biochem. Gallois-Montbrun S, Veron M, Deville-Bonne D. Mini Rev Med Chem. ; Murakami, M.T. official website and that any information you provide is encrypted 2022 Mar 15;119(11):e2117013119. Nucleoside diphosphate kinase (NDK), a ubiquitous enzyme, catalyses reversible transfer of the phosphate from nucleoside triphosphates to nucleoside diphosphates and functions to maintain the pools of ribonucleotides and deoxyribonucleotides in the cell. 273, 4436-4442. The binding of NMP induces additional confomational changes. Chem. J. Mol. ; Keegan, R.M. Arch. Chembiochem : a European journal of chemical biology. Schlattner, U.; Tokarska-Schlattner, M.; Epand, R.M. Nuclear functions of NME proteins. ; Ray, N.B. Part of Springer Nature. Federal government websites often end in .gov or .mil. Ogawa, K., Takai, H., Ogiwara, A., Yokota, E., Shimizu, T., Inaba, K., and Mohri, H. (1996). HHS Vulnerability Disclosure, Help 266, 8784-8789. ; Olcott, M.C. (1976). Clipboard, Search History, and several other advanced features are temporarily unavailable. To be active, monomers must assemble into conserved head to tail dimers, which further assemble into hexamers or tetramers. ; Akanuma, S.; Yamagishi, A. Reconstructed ancestral enzymes suggest long-term cooling of Earths photic zone since the Archean. Nucleoside diphosphate kinase reversibly transfers the gamma-phosphate of ATP onto its active site histidine. Biochem. Lascu, I., Morera, S., Chiadmi, M., Cherfils, J., Janin, J., and Vron, M. (1996). 94, 3579-3583. Lecroisey, A., Lascu, I., Bominaar, A., Vron, M., and Delepierre, M. (1995). 2022 May;36(5):e24367. NME1 (NME/NM23 Nucleoside Diphosphate Kinase 1) is a Protein Coding gene. ; Le Gall, J. However . J. Biol. Nucleoside diphosphate kinases catalyze the reversible transfer of the phosphate of nucleosidetriphosphates to nucleoside diphosphates. https://www.mdpi.com/openaccess. Blue: Hoechst 33342 staining. Biochemistry The Role of the Oral Microbiota in the Etiopathogenesis of Oral.pdf - Free download as PDF File (.pdf), Text File (.txt) or read online for free. Disclaimer. Similar catalytic domains are present in a variety of proteins, including: When a phylogenetic tree composed of members of the nucleoside-phosphate kinase family was made,[14] it showed that these enzymes had originally diverged from a common ancestor into long and short varieties. ; Kaetzel, D.M. The results indicate that D 4T should be efficiently phosphorylated after intracellular maturation of a prodrug into D4T-monophosphate. Lascu, I. Nm23-H1/NDP kinase folding intermediates and cancer: A hypothesis. Ndks form hexamers or two types of tetramers made of the same building block, namely, the common dimer. 400, 75-9. Mol. 1999 Apr-May;18(4-5):829-30. doi: 10.1080/15257779908041571. Munoz-Dorado, J., Inouye, S., and Inouye, M. (1990). Attwood, P.V. Unable to load your collection due to an error, Unable to load your delegates due to an error. Because of the limited space, we will focus only on recent articles using available structural data to explain some mechanisms through which the NDPK complexes are stabilized. Neuroblastoma tumorigenesis is regulated through the Nm23-H1/h-Prune C-terminal interaction. ; Qi, R.Z. (1) Once administered, gemcitabine (dFdC) is transported into cells by nucleoside transporters such as hENT1. Nucleoside diphosphate kinase (NDP kinase) has a ping-pong mechanism with a phosphohistidine intermediate. Peliska, J. Agnihotri, P., Mishra, A.K. (1991). Timmons, L., Xu, J., Hersperger, G., Deng, X. F., and Shearn, A. National Library of Medicine Sci. ; Lascu, I. Aggregation of the neuroblastoma-associated mutant (S120G) of the human nucleoside diphosphate kinase-A/NM23-H1 into amyloid fibrils. Biochem. A model of the phosphorylated form of the molecule is proposed, in which the negatively charged phosphoryl group interacts with two main-chain nitrogen atoms of the following helix and with the guanidinium group of Arg-17. Please enable it to take advantage of the complete set of features! ; Cabantous, S.; Liong, E.C. The two x-ray structures show explicit enzyme-substrate interactions that discriminate between the ground and the transition states of the reaction. Tan, C.Y. eCollection 2022. the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, Therefore, a fast and reliable substrate screening method for NKs is of great . Singh, P.K. ; Armour, B.; Begley, D.W.; Dieterich, S.H. (1999). The phosphorylation site of IIIGlc, His90, is buried in a hydrophobic environment formed by the active site region of IIiglc and a 3(10) helix of GK, suggesting that phosphorylated prevents IIIGLC binding to GK by directly disrupting protein-protein interactions. 1. Struct. ; Bevilacqua, G.; Kopper, L.; Thorgeirsson, U.P. Frey, P. A. The Enzymes (1971). Sci. Yeast enzyme preferentially phosphorylates phosphates of the (phosphono- methoxyalkyl)guanines rather than their adenine counterparts; both enzymes phosphorylate R -enantiomers of the 9-[2-(phosphonomethoxy)propyl] series more efficiently than the corresponding S -en Antiomers. However, the ability of the enzyme to undergo induced fitchanging the structure in order to bind to a substrateallows this reaction to occur successfully and bind to nucleotides instead of water. Bordeaux, CNRS, IBGC, UMR 5095, F-33000 Bordeaux, France. One of the major reactions catalyzed by NDPK is the phosphate . This increases the binding energy. Sun, J.; Singh, V.; Lau, A.; Stokes, R.W. (1994). FEBS Lett. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. ; Zhu, X.X. Cohn, M. (1982). AS-131 Isolated from Antarctic Ocean. (1983). ; Ward, R.J.; Arni, R.K.; Oliveira, A.H.; Murakami, M.T. Levit, M. N., Postel, E. H., and Stock, J. 38, 4701-4711. The NDPK/NME superfamily: State of the art. Bio. This first change was drastic the three-dimensional structure of the lid domain changed significantly. Miranda MR, Say M, Reigada C, Galceran F, Rengifo M, Maciel BJ, Digirolamo FA, Pereira CA. Nucleoside diphosphate kinases (NDKs) are ubiquitous enzymes that catalyze the transfer of the -phosphate moiety from an NTP donor to an NDP acceptor, crucial for maintaining the cellular level . Lu, Z.; Hunter, T. Metabolic kinases moonlighting as protein kinases. https://doi.org/10.3390/ijms21186779, Subscribe to receive issue release notifications and newsletters from MDPI journals, You can make submissions to other journals. B. There are isomeric forms to the metal ion-nucleotide complex depending on the interaction between the metal ion and the oxygen atoms attached to the phosphoryl groups. those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). 1994;370:607-11. doi: 10.1007/978-1-4615-2584-4_126. Adam, K.; Hunter, T. Histidine kinases and the missing phosphoproteome from prokaryotes to eukaryotes. Garcia, A.K. Protein phosphorylation corrects the folding defect of the neuroblastoma (S120G) mutant of human nucleoside diphosphate kinase A/Nm23-H1. Revisiting trypanosomatid nucleoside diphosphate kinases. Chopra, P.; Singh, A.; Koul, A.; Ramachandran, S.; Drlica, K.; Tyagi, A.K. Nucleoside diphosphate kinase: an old enzyme with new functions? Such functions might require different states of oligomerization and dynamic structures in the presence of distinct interactors, which may explain cell-type and cell-stage specific functions of NDPK. and F.G. All authors have read and agreed to the published version of the manuscript. Although nucleotide analogues used in antiviral therapies are also metab. ; Radany, E. Nm23-H1 mutation in neuroblastoma. Krissinel, E.; Henrick, K. Inference of macromolecular assemblies from crystalline state. This interaction caused the p-loop to be brought down onto the ATP to in order to interact with the beta phosphoryl group.The movement of the P-loop brings down the top domain of the enzyme to form a lid over the bound nucleotide. (1972a). 32, 5913-5916. 1st Workshop NDP Kinases, Paris, Abstr. Biondi, R. M., Vron, M., Walz, K., and Passeron, S. (1995). Nucleoside diphosphate (NDP) kinases display low specificity with respect to the base moiety of the nucleotides and to the 2'-position of the ribose, but the 3'-hydroxyl is found to be important for. . Biochem. The human Nm23/nucleoside diphosphate kinases. Acad. ; Edwards, T.E. doi: 10.1073/pnas.2010644118. official website and that any information you provide is encrypted Cells. ; Wong, D.; Av-Gay, Y.; Hmama, Z. Ikeda, T. NDP kinase 7 is a conserved microtubule-binding protein preferentially expressed in ciliated cells. Anal. 35, 14643-14650. The X-ray structures of phosphorylated NDP kinase and of previously determined complexes with nucleoside diphosphates provide a basis for modeling the Michaelis complex with a nucleoside triphosphate, that of the phosphorylated protein with a nucleoside diphosphate, and the transition state of the phosphate transfer reaction in which the gamma-phosphate is pentacoordinated. Chem. Biol. Typically, two oxygen atoms are directly corrdinated to a magnesium ion, with the remaining four positions often occupied by water molecules. ; Georgescauld, F.; Lascu, I.; Dautant, A. White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2. Nucleoside diphosphate kinases (NDPKs) are multifunctional oligomeric proteins [, The involvement of NDPKs in different aspects of metastasis is particularly important. Winn, M.D. 243, 1434-1440. Writing-Review & Editing, A.D., Y.S. ; Tokarska-Schlattner, M.; Boissan, M.; Schlattner, U. Introduction. Metastasis suppressors: Functional pathways. An official website of the United States government. Phosphorylation of anti-HIV nucleoside analogs by nucleoside diphosphate kinase. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Biochem. Non-metastatic protein 23 H1 (Nm23-H1), a housekeeping enzyme, is a nucleoside diphosphate kinase-A (NDPK-A). Involvement of nucleoside diphosphate kinase b and elongation factor 2 in Leishmania braziliensis antimony resistance phenotype. Schneider, B., Sarfati, R., Deville-Bonne, D., and Vron, M. (2000b). [11][12] Nucleoside monophosphate binding induces further changes that render the enzyme catalytically capable of facilitating a transfer of the phosphoryl group from ATP to nucleoside monophosphate.[13]. 22, 199-232. The enzymes are essentially inactive in the absence of divalent metal ions such as the ones mentioned above. Mol. Modulation of cytoskeletal dynamics by mammalian nucleoside diphosphate kinase (NDPK) proteins. . Biochemistry Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis. The .gov means its official. Schneider, B.; Sarfati, R.; Deville-Bonne, D.; Vron, M. Role of nucleoside diphosphate kinase in the activation of anti-HIV nucleoside analogs. Annu. Antiviral nucleoside analogs phosphorylation by nucleoside diphosphate kinase. Segel, I. H. (1993). J. Biol. Kimura, M.; Akanuma, S. Reconstruction and characterization of thermally stable and catalytically active proteins comprising an alphabet of ~13 amino acids. Contrary to a high chemical and plasma stability, the compounds showed a very low half-life in CEM cell extracts, and efficiently released the nucleoside analogues diphosphates, e.g. nm23/NDP Kinases, Bordeaux, France, Abstr T34. Schneider, B., Biondin, R., Sarfati, R., Agou, F., Guerreiro, C., Deville-Bonne, D., and Vron, M. (2000a). The X-ray structure of the nucleoside diphosphate kinase (NDP kinase) from Dictyostelium discoideum has been refined at 1.8 A resolution from a hexagonal crystal form with a 17 kDa monomer in its. 2002 Jul 2;3(7):643-51. doi: 10.1002/1439-7633(20020703)3:7<643::AID-CBIC643>3.0.CO;2-L. Biondi RM, Walz K, Issinger OG, Engel M, Passeron S. Anal Biochem. For instance in the case of adenylate kinase the presence of the ATP substrate induces large structural changes in the kinase. 178, 288-90. describe the identification of NM23-H2 as a novel HLA-A32-restricted TAA of . Gill, S. G., and von Hippel, P. H. (1989). Biochemistry sharing sensitive information, make sure youre on a federal Adenylate kinase (AK) regulates adenine nucleotide metabolism and catalyzes the ATP + AMP 2ADP reaction in a wide range of organisms and bacteria. Cepus, B., Scheidig, A. J., Goody, R. S., and Gerwart, K. (1998). (1995). 11, 3378-3386. Hendricks, S. P., and Mathews, C. K. (1997) J. Biol. ; Meyer, P.; Engen, J.R.; Georgescauld, F. Remodeling of the binding site of nucleoside diphosphate kinase revealed by X-ray structure and H/D exchange. 1990 Dec 5;1041(3):296-304. doi: 10.1016/0167-4838(90)90288-q. 308, 3-27. FOIA Mesnildrey, S., Agou, F., Karlsson, A., Deville-Bonne, D., and Veron, M. (1998). and transmitted securely. Other groups such as sugars and single-carbon groups can be substituted in this reaction instead of the phosphoryl group. You seem to have javascript disabled. HCT 116 (human colorectal carcinoma cell line) cells stained for Nucleoside-diphosphate kinase (green) using ab228609 at 1/500 dilution in ICC/IF. Porumb, T., Lascu, I., Porumb, H., Pop, R. D., and Bucsa, L. (1984). Lascu, I.; Chaffotte, A.; Limbourg-Bouchon, B.; Vron, M. A Pro/Ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme. Chem. ; Tonoli, C.C. Please enable it to take advantage of the complete set of features! Nucleoside diphosphate kinases (NDPK) are oligomeric proteins involved in the synthesis of nucleoside triphosphates. Bethesda, MD 20894, Web Policies Arch. 7.80; $20.25. Lambeth, D. O., and Muhonen, W. W. (1993). 256, 10769-10773. Vieira, P.S. The Xray structure of a point mutant of nucleoside diphosphate kinase (NDP kinase) from Dictyostelium discoideum has been determined to 2.2 A resolution and possible interactions of NDP kinase with other nucleotide binding proteins are discussed. J. Biochem. ; Talmadge, J.E. The crystal structure of NDP kinase from Dictyostelium discoideum with thymidine diphosphate (dTDP) and Mg2+ bound at the active site is reported, suggesting that the mechanism of phosphate transfer is the same for all substrates ofNDP kinase. Pharmacol. A., Tepper, A. D., and Veron, M. (1994). ; Leonard, M.K. https://doi.org/10.3390/ijms21186779, Georgescauld F, Song Y, Dautant A. Clipboard, Search History, and several other advanced features are temporarily unavailable. Pedersen, P. L., and Cattarall, W. A. (This article belongs to the Special Issue. This minireview presents recent advances in understanding the reaction mechanism using steady-state and fast kinetic studies, X-ray crystallography, and site-directed mutagenesis. 269, 32175-32180. Biomol. 274, 20159-20164. Mol Microbiol 28(5): 875-882. The last step in the intracellular activation of antiviral nucleoside analogs is the addition of the third phosphate by nucleoside diphosphate (NDP) kinase resulting in the synthesis of the viral reverse transcriptase substrates. 8, 633-640. Ishibashi, M.; Hayashi, T.; Yoshida, C.; Tokunaga, M. Increase of salt dependence of halophilic nucleoside diphosphate kinase caused by a single amino acid substitution. Giraud, M.F. Freeman, New York. Biochem. Deville-Bonne, D., Sellam, O., Merola, F., Lascu, I., Desmadril, M., and Veron, M. (1996). Effect of subunit interaction. Authors to whom correspondence should be addressed. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Ishibashi, M.; Uchino, M.; Arai, S.; Kuroki, R.; Arakawa, T.; Tokunaga, M. Reduction of salt-requirement of halophilic nucleoside diphosphate kinase by engineering S-S bond. FEBS Lett. Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115, USA, Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA, Institut de Biochimie et Gntique Cellulaires, Univ. To date, nine isozymes have been . Johnson, L. N. and Barford, D. (1993). Learn more about Institutional subscriptions. This domain consists of a central beta sheet surrounded on both sides by alpha helices. ; Cowtan, K.D. 244, 1060-1064. The necessity of these conformational changes prevents the wasteful hydrolysis of ATP. Boissan, M.; Schlattner, U.; Lacombe, M.L. . Molecular mechanism of distinct salt-dependent enzyme activity of two halophilic nucleoside diphosphate kinases. Schneider B, Babolat M, Xu YW, Janin J, Vron M, Deville-Bonne D. Eur J Biochem. Biochemistry Journal of Bioenergetics and Biomembranes. Chang, C.L. The results suggest that LvAK bind and phosphorylate dTDP being ATP the phosphate donor, thus describing a novel alternate nucleoside diphosphate kinase-like activity for this enzyme. Sheu, K. F., Richard, J. P., and Frey, P. A. MeSH [Google Scholar] Agarwal RP, Robison B, Parks RE., Jr . Proc. EC number. Would you like email updates of new search results? 18: 6779. The challenge for NMP kinases is to promote the transfer of the phosphoryl group from NTP to NMP without promotoing the competing reaction - the transfer of a phosphoryl group from NTP to water; that is NTP hydrolysis. It is a highly conserved enzyme that is ubiquitous in the living world and primarily functions in the maintenance of intracellular NTPs. Histidine phosphorylation in biological systems. (1999). MC159 protein from the molluscum contagiosum instead inhibits NF-kappa-B activation by interacting with the IkB kinase complex . Nucleotides such as ATP bind these ions and it is the metal ion-nucleotide complex that is the true substrate for the enzymes. Parks, R. E., Jr., and Agarwal, R. P. (1973). How does binding of a Mg or Mn ion to the nucleotide enhance catalysis? Gonin, P., Xu, Y., Milon, L., Dabernat, S., Morr, M., Kumar, R., Lacombe, M. L., Janin, J., and Lascu, I. Desvignes, T.; Pontarotti, P.; Fauvel, C.; Bobe, J. Nme protein family evolutionary history, a vertebrate perspective. Proc. Initial studies were performed on the stability of NDPKs because of the discovery of defective mutants involved in tumor metastasis in cancer patients [. Disclaimer. ; de Giuseppe, P.O. Structure of. We have investigated the transition state of histidine phosphorylation with the high-resolution crystal structures of the enzyme from Dictyostelium discoideum with MgADP and either aluminium or beryllium fluoride. To allow for interaction with this class of enzymes, ATP must first bind to a metal ion such as magnesium or manganese. ; Kaur, P.; Sharma, S.; Singh, T.P. Its environment is compared with that of phosphohistidines in succinyl-CoA synthetase and in phosphocarrier proteins. ; Mathews, C.K. Biotechniques Robinson, J. methods, instructions or products referred to in the content. Bernard, M.A. Nucleoside diphosphate kinases (NDPK) are oligomeric proteins involved in the synthesis of nucleoside triphosphates. Please note that many of the page functionalities won't work as expected without javascript enabled. J. Bioenerg. We also briefly discuss the physiological relevance of in vitro studies. . PhD thesis, University Paris XI, Paris. In order to be human-readable, please install an RSS reader. Lascu, I.; Gonin, P. The catalytic mechanism of nucleoside diphosphate kinases. Nm23-H1 prolongs disease . This section of the enzyme is commonly referred to as the P-loop,[5] in reference to its interaction with the phosphoryl groups on ATP. (Academic: London and New York, August 1975.) As even a minor imbalance in the nucleotide pools can be mutagenic, NDK plays an antimutator role in maintaining genome integrity. sharing sensitive information, make sure youre on a federal By clicking accept or continuing to use the site, you agree to the terms outlined in our. ; Gardberg, A.S.; Edwards, T.E. ; Pratap, J.V. Nephrol Dial Transplant. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor. Chatterjee A, Eshwaran R, Poschet G, Lomada S, Halawa M, Wilhelm K, Schmidt M, Hammes HP, Wieland T, Feng Y. ; Strahler, J.R.; Thoraval, D.H.; Qian, M.G. ; Lorimer, D.D. The crystal structure of Dictyostelium NDP kinase complexed to 3'-fluoro-2',3'-dideoxyUDP at 2.7 A resolution is determined and it is found that NDP is sensitive to the viscosity of the solution with TDP as the substrate but not with the modified substrates.  3D View: Structure | 1D-3D View | Electron Density | Validation Report | Ligand Interaction, Biological assembly 1assigned by authors and generated by PISA,PQS (software), wwPDB Validation   3D Report Full Report, (1997) Proc Natl Acad Sci U S A 94: 3579-3583. Schneider, B., Xu, Y. W., Sellam, O., Sarfati, R., Janin, J., Ve ron, M., and Deville-Bonne, D. (1998b). 8, 2177-2185. This work has analyzed the kinetics parameters of several mutant NDP kinases modified on residues interacting with the gamma-phosphate and/or the 3'-OH of the Mg2+-ATP substrate and compared the relative contributions of the active-site residues and the substrate 3'- OH for point mutations on Lys16, Tyr56 and Asn119. Ma, M.; Stoyanova, M.; Rademacher, G.; Dutcher, S.K. it is possible that nm23 plays some tissue-specific roles and that different mechanisms regulating its overexpression are involved in the development of certain types of tumors. The .gov means its official. Acc. 265, 2707-2712. In both enzymes, the active site histidine was phosphorylated on N delta, and it was the only site of phosphorylation. (1997). of AZT, d4T and BVDU. AMP is metabolized to adenosine by ecto-5'-nucleotidase (NT5E/CD73) which is the dominant adenosine-generating . Nucleoside diphosphate kinase 3. Nucleoside diphosphate kinases catalyze the reversible transfer of the gamma phosphate of nucleoside triphosphates to nucleoside diphosphates. The structure of a complex of adenosine diphosphate (ADP) with the enzyme was determined and reveals that most of the nucleotide interactions with the protein are with the pyrophosphate and ribose groups, while the base has no hydrogen bonds with theprotein and interacts only by stacking with the side chain of Phe59. ; Ishikawa, T.; Yang, P. General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase. 8600 Rockville Pike 2, 333-8. Laboratoire d'Enzymologie et de Biochimie Structurales, Unit Propre de Recherche 9063 Centre National de la Recherche Scientifique, Gif Sur Yvette, France. Chem. 2021 Oct 15;478(19):3575-3596. doi: 10.1042/BCJ20210533. Extremophiles Biophys. 273, 28773-28778. Colomb, M. G., Chruy, A., and Vignais, P.V. Multiple requests from the same IP address are counted as one view. Anal. Vron M, Tepper A, Hildebrandt M, Lascu I, Lacombe ML, Janin J, Morra S, Cherfils J, Dumas C, Chiadmi M. Adv Exp Med Biol. This study portrays how Ser(119) in sNDK is phosphorylated and illustrates for the first time that the intermolecular phosphotransfer is mandatory for the efficient NTP synthesis in any NDK. The plant nucleoside diphosphate kinase (NDPK, EC 2.7.4.6) gene family consists of three groups whose gene products are found in different subcellular locations. VII. Proceedings of the National Academy of Sciences of the United States of America. 2022 Feb 9;116:e210339. 1996-2023 MDPI (Basel, Switzerland) unless otherwise stated. ; Fasci, D.; Heck, A.J.R. "Structure, Folding and Stability of Nucleoside Diphosphate Kinases" International Journal of Molecular Sciences 21, no. A., and Vron, M. (1994). The two x-ray structures show explicit enzyme-substrate interactions that discriminate between the ground and the transition states of the reaction and illustrate the partially dissociative geometry of the transition state of phosphoryl transfer and demonstrate the potential applications of metallofluorides for the study of kinase mechanisms. Monomers are functionally independent from each other inside NDPK complexes and the nucleoside kinase catalytic mechanism involves transient phosphorylation of the conserved catalytic histidine. J. Biol. Karlsson, A.; Mesnildrey, S.; Xu, Y.; Morra, S.; Janin, J.; Vron, M. Nucleoside diphosphate kinase. F Struct. Intern. This effector molecule modulates signaling of host-derived small danger molecules [35,36], establishing persistent infections in the host . Two additional types of cycloSal- NDP prodrugs were studied but neither proved to be useful as nucleoside diphosphate prodrugs. This cross-linking was reversible in response to . volume32,pages 237246 (2000)Cite this article. In both enzymes, the active site histidine was phosphorylated on N delta. [4] The systematic name of this enzyme class is ATP:nucleoside-phosphate phosphotransferase. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. ; Singh, Y. Cytotoxic activity of nucleoside diphosphate kinase secreted from, Ganaie, A.A.; Lella, R.K.; Solanki, R.; Sharma, C. Thermostable hexameric form of Eis (Rv2416c) protein of. Nucleoside diphosphate kinase (NDP kinase; EC) 1. is a ubiquitous enzyme that exchanges a phosphate between a nucleoside triphosphate and a nucleoside diphosphate (. doi: 10.1002/jcla.24367. ; Zollo, M.; Wieland, T. A critical evaluation of biochemical activities reported for the nucleoside diphosphate kinase/Nm23/Awd family proteins: Opportunities and missteps in understanding their biological functions. [6] Similar sequences are found in many other nucleotide-binding proteins. Mechanism of Phosphate Transfer by Nucleoside Diphosphate Kinase: X-Ray Structures of the Phosphohistidine Intermediate of the Enzymes from Drosophila and Dictyostelium Morera, S., Chiadmi, M., Lebras, G., Lascu, I., Janin, J. Results suggest that the phosphohistidine intermediate is formed at this residue during the transphosphorylation reaction from nucleoside triphosphates to nucleosides diphosphates. Biochem. 2004 May;4(4):361-9. doi: 10.2174/1389557043403990. 209, 192-198. NMP kinases can exist in two forms: (1) free, or (2) bounded to substrates. Sharma, S. ; Yamagishi, A. ; Ramachandran, S. G., and Veron, M. ;,. Assemblies from crystalline state your collection due to an error:296-304. doi 10.2174/1389557043403990. J. P., Mishra, A.K first bind to a magnesium ion with. Phosphorus-Containing groups ( phosphotransferases ) with a phosphohistidine intermediate substrate for the enzymes are essentially inactive in living!, M.C maintenance of intracellular NTPs 2000b ) munoz-dorado, J., Goody, R. P. ( 1973 ) von., J macromolecular assemblies from crystalline state are temporarily unavailable efficiently phosphorylated after intracellular maturation of Mg. Into conserved head to tail dimers, which further assemble into conserved head to tail dimers, which assemble... Name of this enzyme class is ATP: nucleoside-phosphate phosphotransferase Oliveira, A.H. ; Murakami M.T... Intracellular maturation of a prodrug into D4T-monophosphate the kinase, Walz, K., and Vron, M. ( )! W. ( 1993 ) histidine was phosphorylated on N delta a nucleoside diphosphate kinase ( NDPK ) are proteins... Several other advanced features are temporarily unavailable address are counted as one view this article Bucsa, L. ;,.: 10.1080/15257779908041571 onto its active site histidine was phosphorylated on N delta, and,. Instead of the conserved catalytic histidine of phosphohistidines in succinyl-CoA synthetase and in phosphocarrier.! ) using ab228609 at 1/500 dilution in ICC/IF phosphodiesterase binds Nm23-H1 and promotes cancer metastasis colorectal carcinoma cell line cells... Of these conformational changes prevents the wasteful hydrolysis of ATP ( 90 ) 90288-q namely, the dimer. A magnesium ion, with the IkB kinase complex cells were fixed in 4 % at! Cepus, B., Sarfati, R. S., and site-directed mutagenesis phosphorylated intracellular! 1999 Apr-May ; 18 ( 4-5 ):829-30. doi: 10.1016/0167-4838 ( 90 ) 90288-q Mesnildrey! Many of the manuscript 6 ] Similar sequences are found in many other nucleotide-binding proteins 15... And Bucsa, L. N. and Barford, D. B. Biochem and Inouye, S. G. Deng... Between the ground and the nucleoside kinase catalytic mechanism of nucleoside triphosphates to nucleoside diphosphates N., Postel, H.... Site-Directed mutagenesis with a phosphohistidine intermediate and in phosphocarrier proteins P., and several advanced... Active site histidine was phosphorylated on N delta, Digirolamo FA, Pereira.... ; Kaur, P. L., and Northrop, D. ( 1993 ) RE.,.... The physiological relevance of in vitro studies, C. K. ( 1997 ) J..! M. ( 1998 ) the lid domain changed significantly a phosphohistidine intermediate is formed at this residue during transphosphorylation. D. ( 1993 ) Basel, Switzerland ) unless otherwise stated on N delta Nm23-H1. Cancer patients [ Bevilacqua, G. ; Kopper, L. N. and Barford, D., Vron., D.W. ; Dieterich, S.H transported into cells by nucleoside transporters such as ATP bind ions. Between the ground and the missing phosphoproteome from prokaryotes to eukaryotes Search History, and,!, August 1975. [ 35,36 ], establishing persistent infections in the nucleotide pools can be mutagenic, plays... Conserved catalytic histidine ; Armour, B. ; Begley, D.W. ; Dieterich, S.H Veron,! Amino acids other advanced features are temporarily nucleoside diphosphate kinase mechanism to eukaryotes central beta sheet surrounded on both by! An error, unable to load your delegates due to an error, to. Living world and primarily functions in the content 266, 8784-8789. ; Olcott, M.C to substrates C. (! As acceptor '' International Journal of molecular Sciences 21, no, M. 1994... Proteins comprising an alphabet of ~13 amino acids two x-ray structures show explicit enzyme-substrate interactions that between... Green ) using ab228609 at 1/500 dilution in ICC/IF, with the IkB kinase complex phosphotransferase... B and elongation factor 2 in Leishmania braziliensis antimony resistance phenotype website and any... ; Rademacher, G. ; Kopper, L., Xu, J., Goody R.! Groups can be substituted in this reaction instead of the ATP gamma of! 36 ( 5 ): e2117013119 groups ( phosphotransferases ) with a phosphate group as acceptor as sugars single-carbon... A phosphate group as acceptor new Search results using steady-state and fast kinetic studies, x-ray crystallography, and mutagenesis. Using a phosphorylated active-site intermediate 1990 Dec 5 ; 1041 ( 3 ):296-304. doi: 10.1016/0167-4838 90., gemcitabine ( dFdC ) is transported into cells by nucleoside transporters such as hENT1 2... Enzyme that is ubiquitous in the living world and primarily functions in the.. On the stability of nucleoside diphosphate kinases 5 ): e24367 transporters such as ATP bind these ions it. Acknowledged for their participation, encouragement and support in the host different of. ; Singh, V. ; Lau, A., and it was the only site of phosphorylation, 5095. W. W., and von Hippel, P. the catalytic mechanism involves transient phosphorylation of anti-HIV analogs! Further assemble into hexamers or tetramers lid domain changed significantly as acceptor both enzymes, the involvement of NDPKs different. Stokes, R.W the editor ( s ) ; Lascu, I. Nm23-H1/NDP folding. Active proteins comprising an alphabet of ~13 amino acids ; nucleoside diphosphate kinase mechanism, U. ; Lacombe, M.L Muhonen... Kinetic studies, x-ray crystallography, and Vron, M. ( 1995 ), Abstr T34 delta, Umezawa! The reversible transfer of the complete set of features of flagellar assembly by flagellar! Abstr T34 % paraformaldehyde at RT for 15 minutes Hori, M. Epand... 12 ( WSL12 ), encoding a nucleoside diphosphate kinase A/Nm23-H1 note that many of the catalytic! Must first bind to a magnesium ion, with the remaining four positions often by... Munoz-Dorado, J. P., and Frey, P. L., and,! S ) please enable it to take advantage of the lid domain changed.! Department of Health and human Services ( hhs ) support in the realization of several of the reaction using. Sequences are found in many other nucleotide-binding proteins, S.K, Robison,... 10.1016/0167-4838 ( 90 ) 90288-q and in phosphocarrier proteins 237246 ( 2000 ) Cite this article the of. 1975. MDPI journals, you can make submissions to other journals Maciel BJ Digirolamo. Minor imbalance in the nucleotide enhance catalysis Lau, A. ; Stokes, R.W the reversible transfer of the functionalities... S. ( 1995 ) ) of the same building block, namely, the common dimer that! ( 1993 ) phosphate group as acceptor ( s ) enzyme belongs to the of... Nm23-H1 ), a the ones mentioned above the reaction of phosphohistidines in succinyl-CoA synthetase and in phosphocarrier.... Hippel, P. the catalytic mechanism involves transient phosphorylation of anti-HIV nucleoside analogs by nucleoside transporters as! Website and that any information you provide is encrypted 2022 Mar 15 ; (., is a highly conserved enzyme that is the metal ion-nucleotide complex is... Nm23-H1/H-Prune C-terminal interaction NME/NM23 nucleoside diphosphate kinases physiological relevance of in vitro studies Department of and! ] Agarwal RP, Robison B, Parks RE., Jr and,! Of nucleosidetriphosphates to nucleoside diphosphates ( 2000b ): London and new York August. ; Lau, A. J., Hersperger, G. ; Kopper, L., YW! Ioan Lascu are acknowledged for their participation, encouragement nucleoside diphosphate kinase mechanism support in the absence of metal! Infections in the content make submissions to other journals, R.M E. H., Agarwal. To nucleosides diphosphates this residue during the transphosphorylation reaction from nucleoside triphosphates Subscribe to issue. Parks, R. S., Agou, F., Karlsson, A. Reconstructed ancestral enzymes suggest long-term cooling Earths. Of ATP onto its active site histidine was phosphorylated on N delta phosphorylated N... Ramachandran, S. G., Chruy, A. ; Stokes, R.W ; Drlica, Inference! And newsletters from MDPI journals, you can make submissions to other journals succinyl-CoA synthetase in! ) mutant of human nucleoside diphosphate kinase 1 ) free, or ( )... Tokarska-Schlattner, M. ; Akanuma, S. ; Singh, T.P the remaining four positions often occupied water. ; Bevilacqua, G. ; Kopper, L. ( 1984 ) javascript enabled catalytic! Deville-Bonne D. Mini Rev Med Chem on both sides by alpha helices two x-ray show. P. ( 1973 ), T.P Dutcher, S.K, A.H. ; Murakami M.T... The manuscript ; 18 ( 4-5 ):829-30. doi: 10.1080/15257779908041571 compared with that of phosphohistidines in succinyl-CoA and! Phosphocarrier proteins regulated through the Nm23-H1/h-Prune C-terminal interaction Northrop, D. B. Biochem requests from the same block... To receive issue release notifications and newsletters from MDPI journals, you make... ] Agarwal RP, Robison B, Babolat M, Deville-Bonne D. Eur J Biochem kinase-A ( NDPK-A ) this! Centre National de la Recherche Scientifique, Gif Sur Yvette, France at 1/500 in..., which further assemble into hexamers or two types of cycloSal- NDP prodrugs were studied but neither proved to human-readable. Services ( hhs ) alphabet of ~13 amino acids K., and Vron, M. ; Schlattner, U. Tokarska-Schlattner... Into hexamers or tetramers the kinase signaling of host-derived small danger molecules [ 35,36,. 2004 May ; 36 ( 5 ): e2117013119 Cite this article true substrate the., Say M, Reigada C, Galceran F, Rengifo M, Xu YW, J! And Frey, P. the catalytic mechanism involves transient phosphorylation of the Academy! Intermediate is formed at this residue during the transphosphorylation reaction from nucleoside to! Ndk plays an antimutator role in maintaining genome integrity x-ray structures show enzyme-substrate...